Target (protein/gene name): Citrate Synthase NCBI Gene # or RefSeq#: 3266296 Protein ID (NP or XP #) or Wolbachia#: Q5GRQ1 Organism: Wolbachia Etiologic Risk Group (see link below): N/A Background/Disease Information: Essentiality of this protein: Complex of enzymes: EC#: 2.3.3.1 Link to BRENDA EC# page:http://www.brenda-enzymes.org/php/result_flat.php4?ecno=2.3.3.1 Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): -- link to Sigma (or other company) page for assay or assay reagents (substrates):http://www.tdrtargets.org/targets/view?gene_id=232805 -- link to paper that contains assay information: Structure Available (PDB or Homology model) -- PDB # or closest PDB entry if using homology model: -- Query Coverage (if not direct match): -- Max Ident (if not direct match): Druggable Target (see Databases for this): No druggable targets Current Inhibitors: Expression Information (has it been expressed in bacterial cells): Purification Method: Image of protein (PyMol or etc): Amino Acid Sequence: length of your protein in Amino Acids Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website: 46.623 kDa Molar Extinction coefficient of your protein at 280 nm wavelength: CDS Gene Sequence: GC% Content for gene: CDS Gene Sequence (codon optimized) - after having done Primer Design: GC% Content for gene (codon optimized):
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
Primer design results for 'tail' primers (this is just 2 sequences):
NCBI Gene # or RefSeq#: 3266296
Protein ID (NP or XP #) or Wolbachia#: Q5GRQ1
Organism: Wolbachia
Etiologic Risk Group (see link below): N/A
Background/Disease Information:
Essentiality of this protein:
Complex of enzymes:
EC#: 2.3.3.1
Link to BRENDA EC# page: http://www.brenda-enzymes.org/php/result_flat.php4?ecno=2.3.3.1
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):
-- link to Sigma (or other company) page for assay or assay reagents (substrates): http://www.tdrtargets.org/targets/view?gene_id=232805
-- link to paper that contains assay information:
Structure Available (PDB or Homology model)
-- PDB # or closest PDB entry if using homology model:
-- Query Coverage (if not direct match):
-- Max Ident (if not direct match):
Druggable Target (see Databases for this): No druggable targets
Current Inhibitors:
Expression Information (has it been expressed in bacterial cells):
Purification Method:
Image of protein (PyMol or etc):
Amino Acid Sequence:
length of your protein in Amino Acids
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website: 46.623 kDa
Molar Extinction coefficient of your protein at 280 nm wavelength:
CDS Gene Sequence:
GC% Content for gene:
CDS Gene Sequence (codon optimized) - after having done Primer Design:
GC% Content for gene (codon optimized):
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
Primer design results for 'tail' primers (this is just 2 sequences):